Canine Hybloc™ DNA

Canine Hybloc™ DNA is the repetitive sequence fraction of genomic DNA obtained from male canine spleen by extracting, shearing, denaturing, and reannealing DNA under conditions that enrich for repetitive sequences. Canine Hybloc™ DNA may be effectively substituted for any other competitor DNA (e.g., Cot-1 DNA^®, salmon sperm DNA, genomic DNA, etc.) to suppress cross-hybridization to canine repetitive sequences for fluorescence in situ hybridization (FISH), Southern blots, array comparative genomic hybridization (array CGH), hybrid capture and any other protocol requiring competition to block repetitive DNA from binding to the target. Hybridization with Hybloc™ DNA shows a marked increase in signal intensity and a significant decrease in the amount of background noise associated with repetitive DNA.

Cot-1 DNA^® is a registered trademark of Life Technologies Corporation

Canine Hybloc™ DNAs are supplied in 500 µg quantities at 1 mg/mL in 10 mM Tris-HCl (pH 7.4), 1 mM EDTA. Ships on wet ice. Store at -20°C. Avoid repeated freeze/thaw cycles.

Determination of concentration from the A260 and determination of the A260/A280 ratio (1.8 to 2.0); determination of molecular size via gel electrophoresis: ≥80% of Canine Hybloc™ DNA will run as a broad band within the 50 bp-600 bp size range.