Human Female Metaphase – 5 Slides

Human Female Metaphase Slides are prepared using standard cytogenetic slide preparation methods. They are prepared from karyotypically normal female PHA-stimulated peripheral blood lymphocytes that are cultured for approximately 72 hours, synchronized, and then cultured for an additional 5-7 hours before continuing with a standard chromosome harvest protocol. Slides produced are ideal for various FISH protocols, including probe verification and CGH.

Human Female Metaphase Slides are supplied in units of 5 and are ready to hybridize. Each slide can accommodate two 22mm² hybridization areas, for a total of 10 hybridization areas per unit. Store at -20°C for a shelf life of at least 6 months. Shelf life may be extended to 2+ years if stored at -80°C with a desiccant. If stored at room temperature, use within 2 weeks is recommended. Expiration dates are not assigned to this product by AGL due to differences in end-user preferences for storage conditions and slide-aging techniques.

Slides from each lot are stained and examined for the presence of metaphase spreads. Metaphases are examined to ensure that all chromosomes are present, there are no abnormalities and chromosomes have a consistent length of 400-550 bands. Slides are also examined for optimal metaphase spreading, low surrounding cytoplasm and a high (minimum 4%) mitotic index. Each 22mm² hybridization area contains at least 20 high-quality metaphase spreads.